LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1952..2048) /label=attL2 /note="attL2 (c)" misc_signal complement(2063..2082) /note="T7 Promoter/priming site (c)" primer_bind 2090..2106 /note="M13 Reverse priming site" gene 2219..3028 /note="Kanamycin resistance gene" rep_origin 3149..3822 /note="pUC origin" vector join(1960..3825,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1945 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:12650 IMAGE:4127135" /tissue_type="Brain, neuroblastoma" /clone_lib="NIH_MGC_19" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..1945 /gene="MGAT2" /gene_synonym=CDGS2 /gene_synonym=GLCNACTII /gene_synonym=GNT-II /gene_synonym=GNT2 /db_xref="GeneID:4247" /db_xref="HGNC:7045" /db_xref="MIM:602616" CDS 692..1945 /dnas_title="mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase" /gene="MGAT2" /gene_synonym=CDGS2 /gene_synonym=GLCNACTII /gene_synonym=GNT-II /gene_synonym=GNT2 /codon_start=1 /product="mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase" /protein_id="AAH06390.1" /db_xref="GI:13623555" /db_xref="GeneID:4247" /db_xref="HGNC:7045" /db_xref="MIM:602616" /translation="MRFRIYKRKVLILTLVVAACGFVLWSSNGRQRKNEALAPPLLDA EPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLTLRYRSLVYQLNFDQ TLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEI NQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKLGCINAEYPDS FGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMW KLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAYQKLIEC TDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRPSTQS AQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ" misc_feature 692..1945 /note="MGAT2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccgacaaagg aagaacgagg ccctcgcccc 721 accgttgctg gacgccgaac ccgcgcgggg tgccggcggc cgcggtgggg accacccctc 781 tgtggctgtg ggcatccgca gggtctccaa cgtgtcggcg gcttccctgg tcccggcggt 841 cccccagccc gaggcggaca acctgacgct gcggtaccgg tccctggtgt accagctgaa 901 ctttgatcag accctgagga atgtagataa ggctggcacc tgggcccccc gggagctggt 961 gctggtggtc caggtgcata accggcccga atacctcaga ctgctgctgg actcacttcg 1021 aaaagcccag ggaattgaca acgtcctcgt catctttagc catgacttct ggtcgaccga 1081 gatcaatcag ctgatcgccg gggtgaattt ctgtccggtt ctgcaggtgt tctttccttt 1141 cagcattcag ttgtacccta acgagtttcc aggtagtgac cctagagatt gtcccagaga 1201 cctgccgaag aatgccgctt tgaaattggg gtgcatcaat gctgagtatc ccgactcctt 1261 cggccattat agagaggcca aattctccca gaccaaacat cactggtggt ggaagctgca 1321 ttttgtgtgg gaaagagtga aaattcttcg agattatgct ggccttatac ttttcctaga 1381 agaggatcac tacttagccc cagactttta ccatgtcttc aaaaagatgt ggaaactgaa 1441 gcagcaagag tgccctgaat gtgatgttct ctccctgggg acctatagtg ccagtcgcag 1501 tttctatggc atggctgaca aggtagatgt gaaaacttgg aaatccacag agcacaatat 1561 gggtctagcc ttgacccgga atgcctatca gaagctgatc gagtgcacag acactttctg 1621 tacttatgat gattataact gggactggac tcttcaatac ttgactgtat cttgtcttcc 1681 aaaattctgg aaagtgctgg ttcctcaaat tcctaggatc tttcatgctg gagactgtgg 1741 tatgcatcac aagaaaacct gtagaccatc cactcagagt gcccaaattg agtcactctt 1801 aaataataac aaacaataca tgtttccaga aactctaact atcagtgaaa agtttactgt 1861 ggtagccatt tccccaccta gaaaaaatgg agggtgggga gatattaggg accatgaact 1921 ctgtaaaagt tatagaagac tgcagTAGGA CCCAGCTTTC TTGTACAAAG TTGGCATTAT 1981 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT 2041 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT 2101 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT 2161 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT 2221 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT 2281 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT 2341 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA 2401 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC 2461 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG 2521 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC 2581 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG 2641 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC 2701 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA 2761 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA 2821 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC 2881 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT 2941 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT 3001 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA 3061 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG 3121 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 3181 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 3241 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 3301 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 3361 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 3421 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 3481 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 3541 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 3601 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 3661 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 3721 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 3781 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT //